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Isothermal Titration Calorimeter (ITC)

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Isothermal Titration Calorimeter (ITC) at USIC

Requisition forms

Please download the requisition form, fill it in, sign it, and upload the filled copy with your booking. This requisition form is provided by the USIC office for this instrument.

Process: submit booking β†’ in-charge approval β†’ pay on the DU fee portal β†’ upload Order ID + receipt β†’ measurement confirmed. See the payment flowchart and user guidelines.

Booking details

Per sampleβ€”
Total costβ€”

Rates as per the revised USIC user-charges document β€” external rates include 18% GST. Per-hour instruments: enter the number of hours; per-sample instruments: number of samples.

Upload the filled & signed copy of the downloaded requisition form. Only genuine PDF files up to 5 MB are accepted.

Payment is mandatory with every booking. Pay the fee at the DU fee portal β€” fee.du.ac.in β†’ Fee Payment (Miscellaneous) β€” selecting β€œUSIC Instrumentation Fee” under the Fee dropdown. Then enter the Order ID and upload the fee receipt below β€” bookings without payment details are not accepted.

By submitting you accept the USIC user guidelines. Charges are payable in advance and non-refundable.

About this instrument

The TA nanoITC Isothermal Titration Calorimeter (ITC) is a high-precision instrument used to measure the heat changes that occur during chemical reactions, particularly in the fields of biochemistry, molecular biology, and material science. It provides detailed insights into thermodynamic properties such as binding affinities, enthalpy, and entropy, which are critical for understanding molecular interactions and reaction mechanisms.

Features & Specifications:

  1.  
    1. High Sensitivity:
  • As low as 50 nJ per injection, enabling the detection of weak interactions.
    1. Small Sample Volume:
  • The nanoITC is designed for small sample volumes, with as little as 200 Β΅Lrequired for both the sample and reference cells.
    1. Wide Range of Sample Types:
  • The instrument can analyze proteins, nucleic acids, lipids, small molecules, and polymers.
  • The system allows for high-throughput analysis of ligand-bindingand reaction kinetics.
    1. Temperature Control:
  • The nanoITC features precise temperature control, typically ranging from 5Β°C to 80Β°C.
    1. Data Analysis Software:
  • Comes with powerful data analysis softwarethat automates the calculation of thermodynamic parameters, including binding affinity (Ka), enthalpy (Ξ”H), entropy (Ξ”S), and Gibbs free energy (Ξ”G).