High Resolution Mass Spectrometer (HRMS)

Spectroscopy Facilities

Make: Agilent G6530AA (LC-HRMS-Q-TOF)
Source – ESI and APCI source.
Mass accuracy: > 2ppm
HPLC pressure: 600 Bar, MS pressure: 10 -4 to 10 -5 Torr
Resolution: 20,000
Features: Molecular formula determination, accurate molecular weight determination, molecular structure determination, and Reaction kinetic studies.

For any query related to the instrument,
Contact : Mr. Suhail Khan (8126356032)

  • Category Spectroscopy Facilities
  • Availability Available
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Instrument's Description

Liquid chromatography / Mass Spectroscopy (LC/MS) is a technique which combines high performance liquid chromatography HPLC, a powerful analytical separation technique with mass spectroscopy, a powerful analysis & detection technique. There are two common atmospheric pressure ionization (API) LC/MS process: Electrospray Ionization (ESI) & Atmospheric Pressure Chemical Ionization (APCI). Both are soft ionization technique.  Agilent iFunnel technology combines highly efficient electrospray ion generation and focusing of Agilent Jet Stream technology with a hexabore capillary sampling array and dual-stage ion funnel for increased ion sampling and transmission. NanoHPLC combined with mass spectrometer can analysed small molecule as well large molecules like proteins UHPLC separations can be detected by PDA & Mass spectrometer as different detectors.

Sample Description
 Solvent for mobile phase should be mentioned or solubility of compound in volatile solvent
 As HP-LC is available in most departments, LC of submitted samples indicating the operation should be supplied along with the sample.
 At present C8, C18 & others like cyano, amino, Si columns are available. You can submit the interest for separation or repeatability of HP-LC run.
 For HPLC PDA analysis, wavelength should be given.
 Protein samples should be properly digested and desalted.
 Source of the compound should be mentioned.
 MS-DS (Material Safety Data Sheet) should be given along with samples to ensure that there sample being given. Samples should not be toxic or hazardous. Samples will not be accepted unless MS-DS
Price List
For Internal usage Per Sample 65
For External (Academics) usage Per Sample 800
For External (Industries) usage Per Sample 1500